Journal: Scientific Reports

Article Title: Expression and significance of IL-6 and IL-8 in canine mammary gland tumors

doi: 10.1038/s41598-023-28389-3

Figure Lengend Snippet: Serum levels of IL-6, IL-8 and IL-10 in CMGTs and healthy dogs. The serum levels of IL-6 ( A ), IL-8 ( B ) and IL-10 ( C ) in CMGTs and healthy dogs were detected by ELISA assay. One-way analysis of variance (ANOVA) by Tukey’s multiple comparison test was used to analyze multiple group comparisons data. The results from three independent experiments are presented as the mean ± standard deviation (SD). *denotes P < 0.05 and **denotes P < 0.01 compared with the healthy canine mammary gland serum group.

Article Snippet: Immunohistochemical method (IHC) was performed on 3 μm thick sections with the following primary antibodies, such as: polyclonal rabbit anti-IL-6 (dilution, 1:200), polyclonal rabbit aniti-IL-8 (dilution, 1:200) and polyclonal rabbit anti-IL-10 (1:150) (Bioss Biotechnology, Beijing, China).

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Scientific Reports

Article Title: Expression and significance of IL-6 and IL-8 in canine mammary gland tumors

doi: 10.1038/s41598-023-28389-3

Figure Lengend Snippet: Immunohistochemical staining of IL-6, IL-8 and IL-10 in CMGTs and normal canine mammary gland tissues. Representative images (200X) show IHC staining of ( A , D and G ) IL-6, ( B , E and H ) IL-8 and ( C , F and I ) IL-10 in CMGTs and normal canine mammary gland tissue sections. Both cell membrane and cytoplasmic staining is indicated.

Article Snippet: Immunohistochemical method (IHC) was performed on 3 μm thick sections with the following primary antibodies, such as: polyclonal rabbit anti-IL-6 (dilution, 1:200), polyclonal rabbit aniti-IL-8 (dilution, 1:200) and polyclonal rabbit anti-IL-10 (1:150) (Bioss Biotechnology, Beijing, China).

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry

Journal: Scientific Reports

Article Title: Expression and significance of IL-6 and IL-8 in canine mammary gland tumors

doi: 10.1038/s41598-023-28389-3

Figure Lengend Snippet: Expression of IL-6, IL-8 and IL-10 proteins in CMGTs and normal canine mammary gland tissues. ( A–D ) The levels of IL-6, IL-8 and IL-10 protein in CMGTs and normal canine mammary gland tissues were estimated by western blot analysis. β-actin was used as loading control. Uncropped images for Western blots are presented in Supplementary Fig. . Relative protein levels were quantified using ImageJ 1.48 software. Immunoblots were captured to calculate the normalized values presented in the graph by estimation of express levels from IL-6, IL-8 and IL-10 proteins relative to β-actin protein. Data are presented as mean ± SD of triplicate experiments. *denotes P < 0.05 and ** P < 0.01 compared with the healthy canine mammary gland tissues group.

Article Snippet: Immunohistochemical method (IHC) was performed on 3 μm thick sections with the following primary antibodies, such as: polyclonal rabbit anti-IL-6 (dilution, 1:200), polyclonal rabbit aniti-IL-8 (dilution, 1:200) and polyclonal rabbit anti-IL-10 (1:150) (Bioss Biotechnology, Beijing, China).

Techniques: Expressing, Western Blot, Software

Journal: Life

Article Title: Immunomodulatory Effect of Lactobacillus reuteri ( Limosilactobacillus reuteri ) and Its Exopolysaccharides Investigated on Epithelial Cell Line IPEC-J2 Challenged with Salmonella Typhimurium

doi: 10.3390/life12121955

Figure Lengend Snippet: Dot blot analysis of culture supernatants of each experimental group performed in triplicate, spotted onto nitrocellulose membrane for IL-8 detection. Supernatants from individual experimental groups expressing IL-8 on nitrocellulose membrane. Detection was performed with rabbit anti-IL-8 polyclonal antibody obtained from Antibodies-online GmbH, and with CF ® 770 goat anti-rabbit IgG conjugated antibody, obtained from Bio-Connect. The membranes were scanned in an Odyssey Scanner.

Article Snippet: The membranes were then incubated in 1% BSA in Tris-buffered saline containing Tween-20 (0.05%) containing rabbit anti-IL-8 polyclonal antibody (Antibodies-online GmbH, Germany) at a 1:300 dilution, for 1 h at room temperature.

Techniques: Dot Blot, Expressing

Journal: Drug Design, Development and Therapy

Article Title: Molecular Mechanism Underlying Effects of Wumeiwan on Steroid-Dependent Asthma: A Network Pharmacology, Molecular Docking, and Experimental Verification Study

doi: 10.2147/DDDT.S349950

Figure Lengend Snippet: Screening of HUB genes. ( A ) PPI network of WMW against SDA performed by STRING database with the parameters of the organism for “ Homo sapiens ” and the minimum required interaction score≥ 0.9. ( B ) 35 HUB genes network performed by Cytoscape yFiles Radial Layout. Low degree values showed large sizes and bright colors, and small sizes and dark colors for high degree values on the contrary. ( C ) The 10 core targets network, including JUN, MAPK1, IL6, TP53, RELA, HSP90AA1, EGFR, AKT1, CXCL8 , and TNF.

Article Snippet: Rabbit Anti-IL-8/CXCL8 antibody was purchased from Bioss Antibodies (Beijing, China)[bs-0780R].

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Molecular Mechanism Underlying Effects of Wumeiwan on Steroid-Dependent Asthma: A Network Pharmacology, Molecular Docking, and Experimental Verification Study

doi: 10.2147/DDDT.S349950

Figure Lengend Snippet: DC, BC, CC, and Stress Information of HUB Genes

Article Snippet: Rabbit Anti-IL-8/CXCL8 antibody was purchased from Bioss Antibodies (Beijing, China)[bs-0780R].

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Molecular Mechanism Underlying Effects of Wumeiwan on Steroid-Dependent Asthma: A Network Pharmacology, Molecular Docking, and Experimental Verification Study

doi: 10.2147/DDDT.S349950

Figure Lengend Snippet: Molecular Docking Results

Article Snippet: Rabbit Anti-IL-8/CXCL8 antibody was purchased from Bioss Antibodies (Beijing, China)[bs-0780R].

Techniques: Binding Assay

Journal: Drug Design, Development and Therapy

Article Title: Molecular Mechanism Underlying Effects of Wumeiwan on Steroid-Dependent Asthma: A Network Pharmacology, Molecular Docking, and Experimental Verification Study

doi: 10.2147/DDDT.S349950

Figure Lengend Snippet: Molecular docking between CXCL8 and five pivotal ingredients, including quercetin ( A ), beta-sitosterol ( B ), kaempferol ( C ), palmidin A ( D ), and candletoxin A ( E ).

Article Snippet: Rabbit Anti-IL-8/CXCL8 antibody was purchased from Bioss Antibodies (Beijing, China)[bs-0780R].

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Molecular Mechanism Underlying Effects of Wumeiwan on Steroid-Dependent Asthma: A Network Pharmacology, Molecular Docking, and Experimental Verification Study

doi: 10.2147/DDDT.S349950

Figure Lengend Snippet: Correlation heatmap of docking results between the 10 core targets and five pivotal ingredients. The binding energy was marked by different colors, red for higher scores and green for lower scores. It could be observed that all core targets could stably bind with five pivotal ingredients, especially CXCL8 showed the lowest energy.

Article Snippet: Rabbit Anti-IL-8/CXCL8 antibody was purchased from Bioss Antibodies (Beijing, China)[bs-0780R].

Techniques: Binding Assay, Stable Transfection